6BO6
Eubacterium eligens beta-glucuronidase bound to UNC4917 glucuronic acid conjugate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-12-06 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 179.443, 179.443, 133.778 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.368 - 2.801 |
| R-factor | 0.2598 |
| Rwork | 0.258 |
| R-free | 0.29180 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.744 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.370 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.082 | 0.672 |
| Rmeas | 0.085 | |
| Rpim | 0.020 | |
| Number of reflections | 31703 | 4515 |
| <I/σ(I)> | 29.2 | |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 17.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 12% PEG 400, 0.1 M bis-tris pH 6.5 |
