6BNN

Crystal structure of V278E-glyoxalase I mutant from Zea mays in space group P4(1)2(1)2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-11
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	P 41 21 2
Unit cell lengths	69.950, 69.950, 119.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.700 - 1.550
R-factor	0.19097
Rwork	0.189
R-free	0.23152
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5d7z
RMSD bond length	0.017
RMSD bond angle	1.812
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.700	1.640
High resolution limit [Å]	1.550	1.550
Rmeas	0.065	1.653
Number of reflections	43782	6880
<I/σ(I)>	19.3	1.4
Completeness [%]	99.8	98.8
Redundancy	12.9	13.2
CC(1/2)	0.999	0.684

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.7	294	sodium formate 4.2 M pH 9.7 0.5% PEG 4000