6BN0

Avirulence protein 4 (Avr4) from Cladosporium fulvum bound to the hexasaccharide of chitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.11583
Spacegroup name	P 1 21 1
Unit cell lengths	39.864, 41.081, 121.365
Unit cell angles	90.00, 97.87, 90.00

Refinement procedure

Resolution	60.111 - 1.950
R-factor	0.1698
Rwork	0.168
R-free	0.21400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4z4a
RMSD bond length	0.013
RMSD bond angle	1.261
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	60.111	60.111	2.000
High resolution limit [Å]	1.950	8.720	1.950
Rmerge	0.054	0.023	0.612
Rmeas	0.063	0.027	0.716
Number of reflections	28654	360	2110
<I/σ(I)>	13.69	38.19	2.12
Completeness [%]	99.6	98.4	99.8
Redundancy	3.646	3.569	3.72
CC(1/2)	0.999	0.999	0.748

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	0.1 M Tris:HCl, pH 8.5, 30% PEG 4000, 0.8 M LiCl