6BMN
Structure of human DHHC20 palmitoyltransferase, space group P63
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-05 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.979100 |
Spacegroup name | P 63 |
Unit cell lengths | 99.541, 99.541, 158.386 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 86.205 - 2.250 |
R-factor | 0.258 |
Rwork | 0.257 |
R-free | 0.27790 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.480 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.23) |
Phasing software | PHASER (2.7.15) |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 86.210 | 86.210 | 2.320 |
High resolution limit [Å] | 2.250 | 9.000 | 2.250 |
Rmerge | 0.080 | 0.027 | 4.776 |
Rmeas | 0.089 | 0.030 | 5.327 |
Rpim | 0.039 | 0.013 | 2.325 |
Total number of observations | 216086 | ||
Number of reflections | 41855 | 659 | 3867 |
<I/σ(I)> | 12.3 | ||
Completeness [%] | 99.4 | 97.5 | 99.7 |
Redundancy | 5.2 | 4.9 | 5.2 |
CC(1/2) | 0.999 | 0.999 | 0.439 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | 50mM MES, pH5.8, 50mM KH2PO4, 27.7% PEG 300, 25mM DTT, 1.25% 2,5-Hexanediol after 3.5 months. |