6BMM

Structure of human DHHC20 palmitoyltransferase, space group P21

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-12
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.279510
Spacegroup name	P 1 21 1
Unit cell lengths	86.519, 57.700, 94.098
Unit cell angles	90.00, 115.51, 90.00

Refinement procedure

Resolution	28.850 - 2.350
R-factor	0.2424
Rwork	0.241
R-free	0.26030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.425
Data reduction software	XDS
Data scaling software	Aimless (0.5.27)
Phasing software	PHASER (2.7.15)
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.850	28.850	2.440
High resolution limit [Å]	2.350	8.790	2.350
Rmerge	0.120	0.053	2.201
Rmeas	0.129	0.058	2.349
Rpim	0.045	0.021	0.806
Total number of observations	287546
Number of reflections	35217	702	3633
<I/σ(I)>	10
Completeness [%]	99.9	97	99.5
Redundancy	8.2	7.3	8.2
CC(1/2)	0.998	0.999	0.387

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	50mM MES, pH 6.5, 50mM NaH2PO4, 30.3% PEG 300, 50mM DTT, 2.5% 2,5-hexanediol