6BLR

Crystal Structure of IAg7 in complex with insulin mimotope p8E9E6SS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2014-01-24
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9795
Spacegroup name	C 2 2 21
Unit cell lengths	89.736, 111.379, 95.275
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.592 - 1.960
R-factor	0.1734
Rwork	0.171
R-free	0.22770
RMSD bond length	0.007
RMSD bond angle	0.974
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.040
High resolution limit [Å]	1.960	1.960
Rmerge	0.090
Number of reflections	24967
<I/σ(I)>	11.7
Completeness [%]	74.6
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		278	15% PEG4000, 10mM sodium citrate at pH5.0, 100 mM MgCl2