6BL7

S. cerevisiae stu2 coiled coil domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-03-31
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97921
Spacegroup name	P 1 21 1
Unit cell lengths	57.252, 30.112, 72.856
Unit cell angles	90.00, 102.08, 90.00

Refinement procedure

Resolution	49.317 - 2.500
R-factor	0.2603
Rwork	0.257
R-free	0.29200
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.720
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	9566	1530
<I/σ(I)>	18.3	2.3
Completeness [%]	99.0	92.4
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.75	293	13% w/v PEG8000, 200 mM magnesium acetate, 100 mM sodium cacodylate, pH 5.75