6BL5

Head decoration protein from the hyperthermophilic phage P74-26

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2017-03-21
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.979
Spacegroup name	P 63
Unit cell lengths	89.516, 89.516, 31.390
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.841 - 1.690
R-factor	0.1821
Rwork	0.179
R-free	0.21260
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.046
Data reduction software	HKL-3000
Data scaling software	HKL-3000 (v703x)
Phasing software	AutoSol
Refinement software	PHENIX ((1.11.1_2575))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.841	1.740
High resolution limit [Å]	1.690	1.690
Rmerge	0.057	0.824
Rmeas	0.062	0.888
Rpim	0.023	0.328
Number of reflections	16355	1344
<I/σ(I)>	14.3	2.3
Completeness [%]	99.9	100
Redundancy	7.3	7.2
CC(1/2)	0.999	0.810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	0.1 M Tris-HCl pH 7.0, 20% PEG 3350