6BKQ
Crystal structure of the A/Hong Kong/1/1968 (H3N2) influenza virus hemagglutinin E190D mutant in complex with 6'-SLN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-29 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0331 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 208.784, 130.842, 72.179 |
| Unit cell angles | 90.00, 97.90, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.250 |
| R-factor | 0.17917 |
| Rwork | 0.178 |
| R-free | 0.20787 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fnk |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.469 |
| Data scaling software | HKL-2000 (712) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.320 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rpim | 0.040 | 0.300 |
| Number of reflections | 91326 | 8165 |
| <I/σ(I)> | 15.7 | 1.5 |
| Completeness [%] | 99.8 | 98.2 |
| Redundancy | 6.5 | 4.5 |
| CC(1/2) | 1.000 | 0.740 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M sodium cacodylate pH 6.5, 6% PEG 8000, and 39% 2-methyl-2,4-pentanediol |
