6BK7
1.83 Angstrom Resolution Crystal Structure of N-terminal Fragment (residues 1-404) of Elongation Factor G from Enterococcus faecalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-02 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.450, 86.425, 75.442 |
| Unit cell angles | 90.00, 90.17, 90.00 |
Refinement procedure
| Resolution | 28.950 - 1.830 |
| R-factor | 0.19278 |
| Rwork | 0.191 |
| R-free | 0.21734 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vh6 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.371 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MoRDa |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.860 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.046 | 0.793 |
| Rmeas | 0.052 | 0.900 |
| Rpim | 0.025 | 0.423 |
| Number of reflections | 69321 | 3419 |
| <I/σ(I)> | 28.8 | 2.05 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 4.5 | 4.4 |
| CC(1/2) | 0.774 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.3 | 295 | 12.5 mg/mL protein in 0.5 sodium chloride, 0.01 M Tris, pH 8.3 against screen PEGs II (G6), 15% w/v PEG6000, 5% w/v glycerol, cryoprotectant: reservoir |
