6BK5
Inactive choanoflagellate E3 ubiquitin ligase Cbl TKB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.999996 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 115.251, 115.251, 68.777 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.392 - 2.401 |
R-factor | 0.1688 |
Rwork | 0.167 |
R-free | 0.20850 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.849 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.400 | 2.540 |
High resolution limit [Å] | 2.400 | 2.401 |
Rmeas | 0.075 | 0.527 |
Number of reflections | 20844 | 5377 |
<I/σ(I)> | 9.67 | 1.73 |
Completeness [%] | 99.8 | 83.9 |
Redundancy | 2.04 | 2.03 |
CC(1/2) | 0.996 | 0.648 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 2% (w/v) Tacsimate pH 7.0, 10% (w/v) polyethylene glycol 3,350 |