6BK4
Crystal structure of the HR-1 domain of Drosophila caprin in the P212121 space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-07-22 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.050, 60.040, 99.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.270 - 1.800 |
R-factor | 0.2167 |
Rwork | 0.215 |
R-free | 0.24530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4wbe |
RMSD bond length | 0.006 |
RMSD bond angle | 0.687 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA (3.3.22) |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 36.270 | 60.040 | 1.900 |
High resolution limit [Å] | 1.800 | 5.690 | 1.800 |
Rmerge | 0.037 | 0.495 | |
Rmeas | 0.069 | 0.043 | 0.552 |
Rpim | 0.030 | 0.020 | 0.240 |
Total number of observations | 153362 | ||
Number of reflections | 30126 | 990 | 4358 |
<I/σ(I)> | 13.9 | 13.3 | 1.6 |
Completeness [%] | 99.8 | 92.7 | 100 |
Redundancy | 5.1 | 4.3 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 18% PEG2000, 0.1 M BisTris (pH6.8), 5% glycerol |