6BJY

VSV Nucleocapsid with Polyamide Bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-08-10
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0
Spacegroup name	P 2 21 21
Unit cell lengths	75.480, 165.480, 234.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.295 - 3.460
R-factor	0.2681
Rwork	0.266
R-free	0.31070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gic
RMSD bond length	0.003
RMSD bond angle	0.789
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]			3.550
High resolution limit [Å]	3.460	15.470	3.460
Rmerge	0.150	0.100	0.666
Rmeas	0.169	0.113	0.754
Total number of observations	168334
Number of reflections	38377	451	2821
<I/σ(I)>	6.56	11.06	2.72
Completeness [%]	97.6	84.9	99.1
Redundancy	4.386	3.579	4.384
CC(1/2)	0.991	0.984	0.716

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM Sodium Acetate pH 4.5, 0.01% beta-OG, and 6% PEG 3350, 250 mM Sodium Chloride