6BJV
CIRV p19 protein in complex with siRNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 70 |
| Detector technology | PIXEL |
| Collection date | 2015-03-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.586, 46.997, 54.883 |
| Unit cell angles | 109.44, 111.11, 96.78 |
Refinement procedure
| Resolution | 46.807 - 2.198 |
| R-factor | 0.262 |
| Rwork | 0.260 |
| R-free | 0.29710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rpu |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.807 | 2.250 |
| High resolution limit [Å] | 2.198 | 2.200 |
| Rmeas | 0.147 | 0.741 |
| Rpim | 0.092 | 0.478 |
| Number of reflections | 18756 | 10 |
| <I/σ(I)> | 20.7 | 1.84 |
| Completeness [%] | 93.8 | 68.9 |
| Redundancy | 2.2 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 3-12% PEG 1500, 100 mM Sodium Acetate pH 4.6, 10-40 mM MgCl2 |
