6BJH

CIRV p19 mutant T111S in complex with siRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	70
Detector technology	PIXEL
Collection date	2014-12-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 1
Unit cell lengths	45.788, 46.219, 54.181
Unit cell angles	109.85, 112.11, 95.52

Refinement procedure

Resolution	34.037 - 2.580
R-factor	0.227
Rwork	0.224
R-free	0.27800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rpu
RMSD bond length	0.002
RMSD bond angle	0.429
Data reduction software	XDS
Data scaling software	Aimless (0.3.11)
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	34.250	34.250	2.700
High resolution limit [Å]	2.580	8.950	2.580
Rmerge	0.075	0.025	0.251
Rmeas	0.107	0.035	0.355
Rpim	0.075	0.025	0.251
Number of reflections	10184	248	1052
<I/σ(I)>	5.3
Completeness [%]	85.3	85.5	72.5
Redundancy	1.9	2	1.8
CC(1/2)	0.961	0.996	0.462

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	3-12% PEG 1500, 100 mM Sodium Acetate pH 4.6, 10-40 mM MgCl2