6BJG

CIRV p19 mutant T111H in complex with siRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	70
Detector technology	PIXEL
Collection date	2014-12-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 1
Unit cell lengths	46.386, 46.934, 54.623
Unit cell angles	109.33, 111.12, 96.61

Refinement procedure

Resolution	34.826 - 2.290
R-factor	0.2146
Rwork	0.212
R-free	0.25740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rpu
RMSD bond length	0.004
RMSD bond angle	0.559
Data reduction software	XDS
Data scaling software	Aimless (0.3.11)
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	34.940	34.940	2.380
High resolution limit [Å]	2.290	8.890	2.290
Rmerge	0.054	0.028	0.145
Rmeas	0.062	0.032	0.169
Rpim	0.031	0.016	0.086
Number of reflections	15496	272	1368
<I/σ(I)>	13.3
Completeness [%]	87.4	88.9	78.2
Redundancy	3.9	3.9	3.7
CC(1/2)	0.998	0.998	0.977

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	277.15	3-12% PEG 1500, 100 mM Sodium Acetate pH 4.6, 10-40 mM MgCl2