6BIQ
Structure of NlpC2 from Trichomonas vaginalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-01-01 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.820, 99.030, 102.839 |
| Unit cell angles | 115.02, 96.28, 94.28 |
Refinement procedure
| Resolution | 35.695 - 2.300 |
| R-factor | 0.201 |
| Rwork | 0.199 |
| R-free | 0.24140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6bim |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.803 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.900 | 2.370 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rpim | 0.073 | 0.327 |
| Number of reflections | 53546 | 4164 |
| <I/σ(I)> | 6.9 | 2 |
| Completeness [%] | 93.0 | 89.3 |
| Redundancy | 2.9 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2M Ammonium fluoride 20% PEG3350 |
