6BIE

MISREADING CHAPERONE-SUBSTRATE COMPLEXES FROM RANDOM NOISE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-03
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9786
Spacegroup name	P 41 2 2
Unit cell lengths	42.960, 42.960, 258.169
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.540 - 1.770
R-factor	0.17899
Rwork	0.175
R-free	0.24785
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5INA
RMSD bond length	0.011
RMSD bond angle	1.322
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.540	1.800
High resolution limit [Å]	1.769	1.770
Rmerge	0.062	1.340
Number of reflections	25052
<I/σ(I)>	15.3	1.6
Completeness [%]	100.0	99.5
Redundancy	8.7	8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		273	PEG 3000, IMIDAZOLE, ZINC ACETATE, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K