6BI7

Crystal structure of Rev7-WT/Rev3 as a monomer under high-salt conditions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	77
Detector technology	PIXEL
Collection date	2016-03-03
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.976
Spacegroup name	P 31 2 1
Unit cell lengths	89.696, 89.696, 284.841
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	284.840 - 2.800
R-factor	0.2778
Rwork	0.275
R-free	0.32130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bc8
RMSD bond length	0.011
RMSD bond angle	1.527
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	284.840	2.850
High resolution limit [Å]	2.800	2.800
Rpim	0.044	0.168
Number of reflections	33727	1631
<I/σ(I)>	21.1	2.2
Completeness [%]	99.8	99.9
Redundancy	9.6	9.5
CC(1/2)		0.946

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	hRev7/3 at 60 mg/mL in 5 mM HEPES, 100 mM NaCl, 10 mM DTT, pH=7.4 was mixed in a 1:1 ratio with a well solution consisting of 100 mM sodium citrate, 1M LiCl, 7.5% (w/v) PEG6000 at pH=4.75. Crystals were frozen in the reservoir solution with the addition of 20% (w/v) sucrose