6BHQ

Mouse Immunoglobulin G 2c Fc fragment with complex-type glycan

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	80
Detector technology	PIXEL
Collection date	2017-06-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	67.735, 73.399, 118.408
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.082 - 2.050
R-factor	0.2392
Rwork	0.237
R-free	0.25830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2rgs
RMSD bond length	0.020
RMSD bond angle	2.092
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.080	2.120
High resolution limit [Å]	2.050	2.050
Rmerge	0.047	0.489
Rmeas	0.052	0.553
Number of reflections	37350	3533
<I/σ(I)>	16.9
Completeness [%]	99.0	95
Redundancy	4.8	4.2
CC(1/2)	0.999	0.810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291.15	50 mM HEPES, 5% PEG 3.35K, pH 7.0