6BHP
Crystal structure of the Chlamydomonas reinhardtii LCI1 channel
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-08-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.008 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 85.235, 85.235, 209.784 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.753 - 3.209 |
R-factor | 0.2229 |
Rwork | 0.219 |
R-free | 0.29680 |
Structure solution method | SIRAS |
RMSD bond length | 0.002 |
RMSD bond angle | 0.515 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHELXCD |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.310 |
High resolution limit [Å] | 3.200 | 6.890 | 3.200 |
Rmerge | 0.104 | 0.030 | |
Rmeas | 0.110 | 0.032 | |
Rpim | 0.044 | 0.011 | 0.546 |
Total number of observations | 143222 | ||
Number of reflections | 15305 | ||
<I/σ(I)> | 5.1 | ||
Completeness [%] | 100.0 | 99.9 | 100 |
Redundancy | 9.4 | 8.5 | 9.6 |
CC(1/2) | 0.999 | 0.471 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 298 | PEG2000MME, sodium citrate, nickel chloride |