6BGC
The crystal structure of the W145A variant of TpMglB-2 (Tp0684) with bound glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-13 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.257, 61.592, 70.350 |
| Unit cell angles | 80.22, 77.58, 72.72 |
Refinement procedure
| Resolution | 39.728 - 2.082 |
| R-factor | 0.1823 |
| Rwork | 0.180 |
| R-free | 0.21990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jx2 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.698 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 39.730 |
| High resolution limit [Å] | 2.080 |
| Number of reflections | 36146 |
| <I/σ(I)> | 9.8 |
| Completeness [%] | 91.8 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM MES pH 6.0 200 mM NaCl 20% (w/v) PEG 6,000 |
