6BFZ
Crystal structure of enolase from E. coli with a mixture of apo form, substrate, and product form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-01 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.979186 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 111.262, 143.293, 207.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.690 - 2.210 |
| R-factor | 0.17384 |
| Rwork | 0.171 |
| R-free | 0.21820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fym |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.894 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.690 | 2.240 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.036 | 0.332 |
| Rmeas | 0.051 | 0.470 |
| Rpim | 0.036 | 0.332 |
| Number of reflections | 165432 | 7139 |
| <I/σ(I)> | 13.8 | 2.2 |
| Completeness [%] | 99.3 | 87.4 |
| Redundancy | 1.9 | 1.9 |
| CC(1/2) | 0.998 | 0.786 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 2.0 M ammonium sulfate, 0.1 M MES, pH 6.0, 0.1 M sodium/potassium tartrate |
