6BCK

Crystal Structure of Broadly Neutralizing Antibody N49P7 in Complex with HIV-1 Clade AE strain 93TH057 gp120 core.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-31
Detector	DECTRIS PILATUS 300K
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	61.436, 63.867, 255.285
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.261 - 2.700
R-factor	0.228
Rwork	0.225
R-free	0.28470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3tgt 3tnn
RMSD bond length	0.014
RMSD bond angle	1.163
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.261	2.740
High resolution limit [Å]	2.690	2.690
Rmerge	0.131	0.749
Number of reflections	23639
<I/σ(I)>	9.3	1.07
Completeness [%]	80.6	72.2
Redundancy	3.1	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	294	10% PEG 5000 MME 12% isopropanol 0.1 M MES pH 6.5