6BCD
Crystal structure of Rev7-K44A/R124A/A135D in complex with Rev3-RBM2 (residues 1988-2014)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 289.15 |
Detector technology | PIXEL |
Collection date | 2016-11-04 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.976 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 64.580, 64.580, 116.547 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 116.550 - 1.430 |
R-factor | 0.176 |
Rwork | 0.175 |
R-free | 0.19740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ABD (excluding Rev3 peptide) |
RMSD bond length | 0.026 |
RMSD bond angle | 2.367 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 116.550 |
High resolution limit [Å] | 1.430 |
Rmerge | 0.045 |
Rmeas | 0.048 |
Rpim | 0.016 |
Number of reflections | 51936 |
<I/σ(I)> | 41.78 |
Completeness [%] | 98.5 |
Redundancy | 9.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.25 | 289.15 | well solution containing 100 mM sodium citrate, 1.6 M ammonium sulfate, pH=5.25 was mixed at 1:1 ratio with protein at 45 mg/mL in 5 mM HEPES, 100 mM NaCl, 10 mM DTT, pH=7.4 |