6BBT

Structure of the major pilin protein (T-13) from Streptococcus pyogenes serotype GAS131465

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	110
Detector technology	CCD
Collection date	2014-06-18
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	51.772, 114.968, 128.711
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	85.740 - 1.900
R-factor	0.20519
Rwork	0.204
R-free	0.23577
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3b2m
RMSD bond length	0.009
RMSD bond angle	1.426
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER (2.5.6)
Refinement software	REFMAC (5.8.0131)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.740	1.940
High resolution limit [Å]	1.900	1.900
Rpim	0.071	0.762
Number of reflections	61489	3877
<I/σ(I)>	10.4	1.2
Completeness [%]	100.0	100
Redundancy	14.9	14.9
CC(1/2)	0.997	0.461

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.3	291	20% w/v PEG 6000, 0.2 M MOPS pH 7.3