6BBF
The CRAC channel Orai in an open conformation; H206A gain-of-function mutation
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-08-21 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.100000 |
| Spacegroup name | I 41 |
| Unit cell lengths | 262.344, 262.344, 220.443 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.982 - 6.706 |
| R-factor | 0.3145 |
| Rwork | 0.312 |
| R-free | 0.33700 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.163 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 6.820 |
| High resolution limit [Å] | 6.700 | 6.700 |
| Rpim | 0.011 | 0.570 |
| Number of reflections | 13366 | 644 |
| <I/σ(I)> | 92.4 | 1.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 55.8 | 58.7 |
| CC(1/2) | 0.214 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 38% PEG 400 500 mM NaCl 100 mM Tris-HCl |
