6B9A

Hsp90-alpha N-domain bound to NPCA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 2
Unit cell lengths	64.448, 89.385, 99.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.415 - 1.654
R-factor	0.164742267313
Rwork	0.164
R-free	0.19058
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yer
RMSD bond length	0.013
RMSD bond angle	1.677
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.680
High resolution limit [Å]	1.650	1.650
Rmerge	0.081	0.549
Number of reflections	68453
<I/σ(I)>	29.8	1.9
Completeness [%]	99.2	99.5
Redundancy	6.2	6.4
CC(1/2)		0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	277	Bis-Tris Propane pH 6.4, MgCl2, PEG 2000 MME. Soak crystals with NPCA