6B7W

Structure of hen egg-white lysozyme pre-treated with high pressure (600 MPa) under isobaric condition

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-25
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.459
Spacegroup name	P 43 21 2
Unit cell lengths	78.540, 78.540, 36.946
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.360 - 1.482
R-factor	0.1588
Rwork	0.157
R-free	0.18730
Structure solution method	SAD
RMSD bond length	0.019
RMSD bond angle	1.745
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	39.270
High resolution limit [Å]	1.480
Rmerge	0.029
Rmeas	0.030
Number of reflections	19678
<I/σ(I)>	74.09
Completeness [%]	99.7
Redundancy	21.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.9M sodium chloride, 0.1M sodium acetate pH 4.8