6B7W
Structure of hen egg-white lysozyme pre-treated with high pressure (600 MPa) under isobaric condition
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE W01B-MX2 |
Synchrotron site | LNLS |
Beamline | W01B-MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-05-25 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.459 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 78.540, 78.540, 36.946 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.360 - 1.482 |
R-factor | 0.1588 |
Rwork | 0.157 |
R-free | 0.18730 |
Structure solution method | SAD |
RMSD bond length | 0.019 |
RMSD bond angle | 1.745 |
Data scaling software | XSCALE |
Phasing software | SHELX |
Refinement software | PHENIX |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 39.270 |
High resolution limit [Å] | 1.480 |
Rmerge | 0.029 |
Rmeas | 0.030 |
Number of reflections | 19678 |
<I/σ(I)> | 74.09 |
Completeness [%] | 99.7 |
Redundancy | 21.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.9M sodium chloride, 0.1M sodium acetate pH 4.8 |