6B7U
Structure of hen egg-white lysozyme without high-pressure pre-treatment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-25 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.459 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.079, 78.079, 36.945 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.395 - 1.581 |
| R-factor | 0.172 |
| Rwork | 0.170 |
| R-free | 0.21050 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.366 |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | PHENIX (1.8.3) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 39.040 |
| High resolution limit [Å] | 1.580 |
| Rmerge | 0.045 |
| Number of reflections | 15337 |
| <I/σ(I)> | 38.9 |
| Completeness [%] | 94.9 |
| Redundancy | 14.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.7M sodium chloride, 0.1M sodium acetate pH 4.8 |
