6B7Q
Crystal structure of Legionella effector protein sdeA (lpg2157) aa. 211-910
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.806, 80.616, 85.612 |
| Unit cell angles | 90.00, 109.87, 90.00 |
Refinement procedure
| Resolution | 80.510 - 2.200 |
| R-factor | 0.19731 |
| Rwork | 0.195 |
| R-free | 0.24078 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.813 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 80.510 |
| High resolution limit [Å] | 2.200 |
| Number of reflections | 18728 |
| <I/σ(I)> | 7.98 |
| Completeness [%] | 99.3 |
| Redundancy | 3.278 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 298 | PEG 8,000 9%, 0.1M Hepes pH7.6 |
