6B7O
Crystal structure of Legionella effector sdeD (lpg2509) H67A in complex with ADP-ribosylated Ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-02 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.677, 58.768, 75.111 |
| Unit cell angles | 90.00, 114.42, 90.00 |
Refinement procedure
| Resolution | 68.390 - 1.850 |
| R-factor | 0.16119 |
| Rwork | 0.159 |
| R-free | 0.20077 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.965 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 68.390 |
| High resolution limit [Å] | 1.850 |
| Number of reflections | 43941 |
| <I/σ(I)> | 19.28 |
| Completeness [%] | 100.0 |
| Redundancy | 6.413 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 100 mM Na Cacodylate pH 7.0, 21% PEG 8000 |
