6B7M
Crystal structure of Legionella effector sdeD (lpg2509) in complex with Ubiquitin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-17 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9789 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 64.765, 58.593, 74.124 |
Unit cell angles | 90.00, 114.66, 90.00 |
Refinement procedure
Resolution | 67.360 - 1.700 |
R-factor | 0.1618 |
Rwork | 0.160 |
R-free | 0.19565 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.022 |
RMSD bond angle | 2.033 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 67.360 |
High resolution limit [Å] | 1.700 |
Number of reflections | 108100 |
<I/σ(I)> | 25.4 |
Completeness [%] | 99.4 |
Redundancy | 3.36 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 200 mM NaCl, 100 mM Imidazole pH 7.0, 24% PEG 8000 |