6B64

IMPase (AF2372) with 25 mM Asp

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-09-19
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.0
Spacegroup name	P 32
Unit cell lengths	89.530, 89.530, 102.136
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.648 - 2.600
R-factor	0.173
Rwork	0.170
R-free	0.22150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lvb
RMSD bond length	0.031
RMSD bond angle	1.155
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall
Low resolution limit [Å]	42.650
High resolution limit [Å]	2.600
Number of reflections	28144
<I/σ(I)>	3.444
Completeness [%]	100.0
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	100 mM myo inositol 400 mM Aspartate 15 % PEG 3350 200 mM potassium sulfate 100 mM magnesium chloride 100 mM Hepes 7.0