6B5S

Structure of PfCSP peptide 25 with human antibody CIS42

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-12
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	41.960, 70.817, 164.901
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.422 - 1.983
R-factor	0.1694
Rwork	0.168
R-free	0.20390
RMSD bond length	0.005
RMSD bond angle	0.767
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall
Low resolution limit [Å]	43.422
High resolution limit [Å]	1.980
Number of reflections	34887
<I/σ(I)>	13.4
Completeness [%]	100.0
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	15% PEG3350, 9% Isopropanol, 0.12M Ammonium Citrate pH 8.5