6B4G

Crystal structure of Chaetomium thermophilum Gle1 CTD-Nup42 GBM complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0332
Spacegroup name	P 21 21 21
Unit cell lengths	84.482, 93.055, 229.669
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.864 - 2.648
R-factor	0.2412
Rwork	0.240
R-free	0.27680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.033
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((dev_2006: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.900	2.730
High resolution limit [Å]	2.648	2.650
Rpim	0.026	0.450
Number of reflections	53442	5142
<I/σ(I)>	20.5	1.4
Completeness [%]	99.8	97.6
Redundancy	13.1	10.5
CC(1/2)	1.000	0.669

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	0.1M MES pH 6.3, 12% (w/v) PEG 20,000