6B4C

Structure of Viperin from Trichoderma virens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	77
Detector technology	CCD
Collection date	2015-10-25
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.786
Spacegroup name	P 21 21 21
Unit cell lengths	173.884, 209.566, 233.941
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.814 - 2.795
R-factor	0.1904
Rwork	0.190
R-free	0.21070
Structure solution method	SAD
RMSD bond length	0.015
RMSD bond angle	1.457
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.795	2.795
Rmerge	0.082
Number of reflections	209265
<I/σ(I)>	9
Completeness [%]	99.9
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	1.6-1.8 M ammonium sulfate, 100 mM sodium citrate, pH 5.5-5.7