6B28

Crystal structure of human STAC2 Tandem SH3 Domains Q347I (296-411)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	200
Detector technology	IMAGE PLATE
Collection date	2017-08-28
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54179
Spacegroup name	P 21 21 21
Unit cell lengths	35.383, 49.707, 89.667
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.830 - 2.550
R-factor	0.2233
Rwork	0.222
R-free	0.25170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.535
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.830	35.000	2.630
High resolution limit [Å]	2.550	5.830	2.550
Rmerge	0.135	0.046	0.769
Rmeas	0.158	0.055	0.915
Rpim	0.082	0.030	0.489
Number of reflections	5536	516	432
<I/σ(I)>	6.5
Completeness [%]	99.3	97.5	98.6
Redundancy	3.4	3.2	3.1
CC(1/2)		0.996	0.707

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.4	277.15	0.1M Bis-Tris, 0.2M lithium sulfate, 35% PEG3350