6B26

Crystal structure of human STAC2 Tandem SH3 Domains (296-411)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	36.857, 47.993, 73.587
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.950 - 1.200
R-factor	0.1532
Rwork	0.152
R-free	0.16910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2dl4
RMSD bond length	0.007
RMSD bond angle	1.321
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.950	1.240
High resolution limit [Å]	1.200	1.200
Rmerge	0.024	0.201
Number of reflections	41292
<I/σ(I)>	29.89	5.84
Completeness [%]	99.3	98.95
Redundancy	4.4	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	7.4	277.15	150mM KCl, 10mM HEPES, 2mM TCEP