6B21

Crystal structure of AmtB from E. coli bound to TopFluor cardiolipin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2017-07-29
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	H 3 2
Unit cell lengths	138.780, 138.780, 159.140
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	56.219 - 2.450
R-factor	0.1719
Rwork	0.170
R-free	0.21250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1u7g
RMSD bond length	0.002
RMSD bond angle	0.618
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	56.219	56.219	2.510
High resolution limit [Å]	2.450	10.960	2.450
Rmerge	0.065	0.019	0.490
Rmeas	0.073	0.022	0.559
Number of reflections	21583	260	1590
<I/σ(I)>	14.72	48.94	2.85
Completeness [%]	98.7	93.2	98.9
Redundancy	4.547	4.331	4.168
CC(1/2)	0.999	1.000	0.890

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8		0.1M Tris, pH 8.0 0.3M Mg-nitrate 22% (w/v) PEG 8000