6B1I
Disrupted hydrogen bond network impairs ATPase activity in an Hsc70 cysteine mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-04 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.977 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.830, 79.196, 76.252 |
| Unit cell angles | 90.00, 101.45, 90.00 |
Refinement procedure
| Resolution | 40.727 - 2.300 |
| R-factor | 0.1935 |
| Rwork | 0.191 |
| R-free | 0.24220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4h5r |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.471 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.500 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 40377 | |
| <I/σ(I)> | 10.8 | |
| Completeness [%] | 91.4 | |
| Redundancy | 4.3 | |
| CC(1/2) | 0.993 | 0.891 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | NaCl, Tris, PEG 3350, MgCl2, ATP |
