6B0O

Zinc finger Domain of WT1(-KTS form) with 12+1mer Oligonucleotide with 3' Triplet TGT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2016-11-29
Detector	RAYONIX MX300-HS
Wavelength(s)	1.00000
Spacegroup name	P 1
Unit cell lengths	36.927, 42.657, 71.821
Unit cell angles	88.28, 77.71, 85.81

Refinement procedure

Resolution	29.588 - 1.552
R-factor	0.1933
Rwork	0.193
R-free	0.20620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5kl2
RMSD bond length	0.003
RMSD bond angle	0.528
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.590	1.610
High resolution limit [Å]	1.550	1.550
Rmerge	0.044	0.427
Rpim	0.028	0.327
Number of reflections	59787	5883
<I/σ(I)>	19.1	2.5
Completeness [%]	96.8	95
Redundancy	3.3	3.3
CC(1/2)		0.793

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	289	25% PEG 3350 0.2M Ammonium Acetate 0.1M Bis-Tris