6B0N
Crystal structure of the cleavage-independent prefusion HIV Env glycoprotein trimer of the clade A BG505 isolate (NFL construct) in complex with Fabs PGT122 and PGV19 at 3.39 A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2015-10-18 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.03321 |
Spacegroup name | P 63 |
Unit cell lengths | 161.247, 161.247, 245.651 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.854 - 3.400 |
R-factor | 0.3358 |
Rwork | 0.335 |
R-free | 0.34640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4tvp |
RMSD bond length | 0.014 |
RMSD bond angle | 1.726 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: 000)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.510 |
High resolution limit [Å] | 3.390 | 3.390 |
Rpim | 0.145 | 0.559 |
Number of reflections | 49063 | 4971 |
<I/σ(I)> | 6.9 | 1.3 |
Completeness [%] | 98.7 | 99.9 |
Redundancy | 6.4 | 6.1 |
CC(1/2) | 0.715 | 0.202 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.1M HEPES (pH 7.0), 15% (w/v) PEG4000, and cryo-protected with 25% glycerol |