6AZC

Crystal structure of Physcomitrella patens KAI2-like E S166A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-08-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000
Spacegroup name	P 1 21 1
Unit cell lengths	45.932, 121.821, 46.909
Unit cell angles	90.00, 91.54, 90.00

Refinement procedure

Resolution	46.892 - 2.000
R-factor	0.262794476922
Rwork	0.220
R-free	0.25338
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6azb
RMSD bond length	0.003
RMSD bond angle	0.591
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.900	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.077	0.132
Rpim	0.047	0.079
Number of reflections	35204	3382
<I/σ(I)>	11.52	8.12
Completeness [%]	98.7	97.66
Redundancy	3.7	3.8
CC(1/2)	0.992	0.966

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	0.1 M Tris, pH 8.5, 30% PEG8000