6AZC
Crystal structure of Physcomitrella patens KAI2-like E S166A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-08-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.932, 121.821, 46.909 |
| Unit cell angles | 90.00, 91.54, 90.00 |
Refinement procedure
| Resolution | 46.892 - 2.000 |
| R-factor | 0.262794476922 |
| Rwork | 0.220 |
| R-free | 0.25338 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6azb |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.591 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.900 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.077 | 0.132 |
| Rpim | 0.047 | 0.079 |
| Number of reflections | 35204 | 3382 |
| <I/σ(I)> | 11.52 | 8.12 |
| Completeness [%] | 98.7 | 97.66 |
| Redundancy | 3.7 | 3.8 |
| CC(1/2) | 0.992 | 0.966 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.1 M Tris, pH 8.5, 30% PEG8000 |
