6AYM
Crystal structure of Campylobacter jejuni 5'-methylthioadenosine/S-adenosyl homocysteine nucleosidase (MTAN)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-14 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.408, 84.620, 124.881 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.050 - 1.250 |
| R-factor | 0.19041 |
| Rwork | 0.190 |
| R-free | 0.20532 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wkn |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.335 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.050 | 1.270 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Number of reflections | 134158 | 6445 |
| <I/σ(I)> | 14.8 | 1.9 |
| Completeness [%] | 98.3 | 96.5 |
| Redundancy | 7.3 | 7.4 |
| CC(1/2) | 0.990 | 0.650 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 100 mM MES pH 6.5, 600 mM Sodium Chloride, 20% (w/v) PEG 4000 |
