6AYA

Structure of the native full-length HIV-1 capsid protein in complex with Nup153 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2016-12-01
Detector	RDI CMOS_8M
Wavelength(s)	1.000031
Spacegroup name	P 6
Unit cell lengths	92.544, 92.544, 58.288
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.241 - 2.400
R-factor	0.2358
Rwork	0.234
R-free	0.27360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xfx
RMSD bond length	0.002
RMSD bond angle	0.508
Data reduction software	Aimless (0.5.32)
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER (2.7.17)
Refinement software	PHENIX (1.11.1-2575_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	36.241	36.240	2.490
High resolution limit [Å]	2.400	8.980	2.400
Rmerge	0.065	0.032	0.689
Rmeas	0.068	0.034	0.723
Rpim	0.021	0.011	0.220
Number of reflections	10941	220	1168
<I/σ(I)>	24.7
Completeness [%]	97.2	94.2	100
Redundancy	10.6	9.8	10.8
CC(1/2)	0.999	0.998	0.901

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	PEG3350, NaI, MIB, Glycerol