6AY9

Structure of the native full-length HIV-1 capsid protein in complex with CPSF6 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033203
Spacegroup name	P 6
Unit cell lengths	92.675, 92.675, 58.042
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.030 - 2.500
R-factor	0.2438
Rwork	0.242
R-free	0.27270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xfx
RMSD bond length	0.002
RMSD bond angle	0.496
Data reduction software	Aimless (0.5.32)
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER (2.7.17)
Refinement software	PHENIX (1.11.1-2575_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.030	47.030	2.600
High resolution limit [Å]	2.500	9.010	2.500
Rmerge	0.063	0.033	0.700
Rmeas	0.069	0.037	0.774
Rpim	0.029	0.017	0.326
Total number of observations	55693
Number of reflections	9956	233	1119
<I/σ(I)>	18.1
Completeness [%]	99.9	99.6	100
Redundancy	5.6	5.4	5.5
CC(1/2)	0.998	0.998	0.583

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	PEG3350, NaI, Sodium cacodylate, Glycerol