6AXY

Structure of the V11I/T58A/I124A mutant of the HIV-1 capsid protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2016-12-01
Detector	RDI CMOS_8M
Wavelength(s)	1.000031
Spacegroup name	P 6
Unit cell lengths	91.915, 91.915, 57.919
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.960 - 2.780
R-factor	0.2156
Rwork	0.213
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdbid 4XFX
RMSD bond length	0.007
RMSD bond angle	1.085
Data reduction software	Aimless (0.5.32)
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER (2.7.17)
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.960	45.960	2.930
High resolution limit [Å]	2.780	8.780	2.780
Rmerge	0.109	0.036	1.050
Rmeas	0.115	0.038	1.106
Rpim	0.035	0.012	0.345
Total number of observations	73644
Number of reflections	6969	244	1008
<I/σ(I)>	18
Completeness [%]	97.3	98.7	97.3
Redundancy	10.6	10.3	9.9
CC(1/2)	0.999	0.999	0.754

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	PEG3350, NaI, Sodium cacodylate, Glycerol