6AXN
F95C Epi-isozizaene synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-18 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.18 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.614, 46.787, 75.371 |
| Unit cell angles | 90.00, 98.16, 90.00 |
Refinement procedure
| Resolution | 32.530 - 1.900 |
| R-factor | 0.159 |
| Rwork | 0.157 |
| R-free | 0.19060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kb9 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.461 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1839) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 32.530 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 28111 |
| <I/σ(I)> | 9.9 |
| Completeness [%] | 98.8 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.2 M MgCl2.6H2O, 0.1 M bis-Tris (pH 6.5), 25% (w/v) PEG 3350 |
