6AXM
F95Y Epi-isozizaene synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-11 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.120, 46.830, 75.720 |
| Unit cell angles | 90.00, 96.89, 90.00 |
Refinement procedure
| Resolution | 51.740 - 1.800 |
| R-factor | 0.172 |
| Rwork | 0.170 |
| R-free | 0.21410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kb9 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.830 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 51.740 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 32978 |
| <I/σ(I)> | 7 |
| Completeness [%] | 97.6 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.17 M sodium acetate trihydrate, 85 mM sodium cacodylate trihydrate (pH 6.5), 25.5% PEG 8000, 15% glycerol |
